Supplementary Figure 1: Detection and characterization of remodeler-binding sites.
From: Overlapping chromatin-remodeling systems collaborate genome wide at dynamic chromatin transitions
(a) Left-side, Western blot detection of Brg1 in nuclear extracts with monoclonal antibody. Right-side, top graph, ChIP-QPCR analysis of initial Brg1 antiserum at regions bound by Brg1 (Orm2 and Fabp4) and not bound by Brg1 (tRNA). Right-side, bottom graph, ChIP-QPCR analysis of final monoclonal Brg1 antibody at the same regions described above. (b) Specificity of Brg1, Chd4 and Snf2h antibodies by Western blot analysis of whole cell extracts. MW, molecular weight markers. Asterisk, unidentified band that does not appear following immunoprecipitation with the Chd4 antibody. (c) Additional examples of ChIP-seq genome browser views of Brg1, Chd4, and Snf2h occupancy. Images represent tag densities relative to genome coordinates. Input tracks of sonicated, genomic DNA are aligned below each ChIP-seq track. (d) Box plots of tag density enrichment (log2 scale) of remodeler binding sites located at the indicated genomic regions. Sites are classified as promoter (< 2.5 kb upstream or downstream from the nearest TSS), exon (> 2.5 kb downstream from the nearest TSS , overlapping an exon), intron (> 2.5 kb downstream from the nearest TSS , in an intron not overlapping an exon), distal upstream (> 2.5 kb upstream from the nearest TSS), or downstream (> 2.5 kb downstream from the nearest TSS).
