Supplementary Figure 4: Human Polɛ is not able to proceed through CPD Thy adducts and (6-4)pp lesions.
From: Repriming of DNA synthesis at stalled replication forks by human PrimPol
(a) Left, primer extension assay using purified human Pole on a control template. The full extension product is 27 nucleotides longer than the original primer. Right, primer extension assay using a CPD-containing template. The position of the CPD (T=T) is indicated in red. Polɛ could only extend the primer by 3 nucleotides (TAT, shown in blue) before stopping at the lesion. dNTP concentrations used: 1, 5, 10 μM. (b) Left, primer extension assay using another control template in which the full extension product is 17 nucleotides longer than the original primer. Right, similar assay using a (6-4)pp-containing template. The position of the (6-4)pp is indicated in red. As in (a), Polɛ was unable to bypass the lesion, extending the primer by only 3 nucleotides (ATG, shown in blue). dNTP concentrations used: 1, 5, 10 μM. Data are representative of 4 experiments (a,b).
