Supplementary Figure 2: Two E2P crystal forms of LpCopA.
From: Copper-transporting P-type ATPases use a unique ion-release pathway
(a-d), Crystal packing with the domains are colored as in Fig. 1b. The proteins are arranged as stacked bilayers, held together by hydrophobic interactions between their membrane-spanning regions, typical of the HiLiDe crystallization method41. (a), View along the membrane bilayer for the C2 (high-resolution) crystal form. (b), Equivalent view for the P212121 crystal form. Approximate perpendicular views of (a) and (b) are shown in (c) and (d), respectively. Note the more loose packing of the C2 lattice. The unit cell parameters can be found in Table 1. (e), Electron density maps of the low resolution P212121 form of the E2-BeF3- complex. The search model is based on the high-resolution structure (C2 space group) and is colored as in Fig. 1b. The 2mFo-DFc (blue, 1σ contour level) and mFo-DFc (±14σ contour levels, green and red, respectively) electron density maps were derived upon rigid body refinement and indicate no major deviations from the high-resolution structure
