Supplementary Figure 5: Nascent-RNA sequencing reveals Pol II kinetics. | Nature Structural & Molecular Biology

Supplementary Figure 5: Nascent-RNA sequencing reveals Pol II kinetics.

From: Transcription-generated torsional stress destabilizes nucleosomes

Supplementary Figure 5

(a,b) Genes were separated by increased (red), decreased (blue), or no change (black) in expression relative to control samples after Topo I (a) or II (b) inhibition, and the average change in nascent RNA surrounding the TSS and TES relative to control is plotted. Despite the increase in nascent RNA near the TSS after Topo I inhibition, only a fraction of genes increase in expression as measured by the increased nascent RNA levels at the TES of genes. (c) Heat map of the change in nascent RNA relative to control samples for Topo I (left) and II (right) with genes arranged by change in stalled Pol II. Topo I inhibition results in altered Pol II initiation and elongation kinetics. Ctl: control.

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