Supplementary Figure 4: Full gene visualization of Stau1-binding sites in 3' UTRs, extended 3' UTRs and introns.
From: Staufen1 senses overall transcript secondary structure to regulate translation
RNA-Seq (green) corresponds to sequencing of poly(A) selected total RNA. Stau1-WT CROSS (yellow) corresponds to FLAG-Stau1-WT tandem IPs from formaldehyde crosslinked cells, sonicated to shear Stau1 bound RNAs. Stau1-Mut CROSS (blue) corresponds to the same condition described above performed on cells expressing a mutant Stau1 lacking dsRNA binding activity. Stau1-WT FOOT (brown) corresponds to FLAG-Stau1-WT tandem IP under native conditions that are incubated with RNaseI in order to obtain a footprint of Stau1 bound RNAs. Stau1-Mut FOOT (violet) corresponds to the same condition described above performed on cells expressing a mutant Stau1 lacking dsRNA binding activity. PAS-Seq (black) corresponds to “polyadenylation site” sequencing reads from HEK293 cells.
