Supplementary Figure 8: Quantitative RT-PCR of Stau1-target mRNAs in HEK293, Huh7 and SK-Hep1 cells.
From: Staufen1 senses overall transcript secondary structure to regulate translation
a, Quantitative RT-PCR against Stau1 mRNA targets from HEK293 cells transfected with a control shRNA or with an shRNA targeting Stau1 or with a control shRNA and the addition of doxycycline to overexpress Flag-Stau1 WT. b and c, Quantitative RT-PCR against Stau1 and Stau2 as well as Stau1 targets from Huh7 and SK-Hep1 cells transfected with shRNAs against Stau1 or siRNA against Stau2 or both. Error bars correspond to the standard deviation calculated from 3 biological replicates.
