Supplementary Figure 1: Tandem affinity purification of Stau1 complexes.
From: Staufen1 senses overall transcript secondary structure to regulate translation
a, Western-blotting for endogenous hnrnp-A1 and Stau1 from total cell lysate and from proteins extracted from oligo(dT) pull-down of mRNA after cells were exposed to increasing doses of UV wave-lengths. b. Quantification of exogenous P32-labeled Arf1 mRNA eluted from Flag-Only, Flag-Stau1-Mut and Flag-Stau1-WT FLAG-immunoprecipitates. c, Western blotting for endogenous and Flag-Stau1 proteins during native tandem affinity purifications. d, Same as “c,” for Crosslinked tandem affinity purifications. e, Scatter plots of gene read counts between biological replicates of Stau1-WT and Mut CROSS libraries.
