Supplementary Figure 3: Flow chart of RELION-based hierarchical classification and Fourier shell correlation (FSC) curves for reconstructions from the three classes.
From: EttA regulates translation by binding the ribosomal E site and restricting ribosome-tRNA dynamics
(a) RELION-based classification diagram. See computational classification of Supplementary Note for details. (b) FSC curve to determine the resolution of the reconstructions from the three classes. For each class, the two half-volumes were generated using RELION. The FSC between the two half-volumes was calculated using SPIDER (Online Methods). The resolution of each map was determined using FSC = 0.143 cutoff, yielding 7.5 Å (70S–EttA–tRNA2, Class I), 9.1 Å (70S–EttA–tRNA, Class II) and 7.7 Å (70S–EttA, Class III), respectively.
