Supplementary Figure 2: Data interpretation.
From: Structural basis of lipid-driven conformational transitions in the KvAP voltage-sensing domain
1. The tilt of S4 from PC:PG to DoTAP shown by the vector presentation ΠNiEdda. ΠNi in DOTAP was mapped onto crystal structure with linear blue scale. There is no water accessibility to the center of membrane. The net difference of ΠNi on extracellular and intracellular ends clearly indicates a tilt trend in the direction opposite to the ΠNi. 2. Coupling of sensor, pore and the ion conduction. KvAP G-V curves (c) with different non-phosphate lipid contents (50%, 67% and 80%) were simulated with published Vh (b) and z values. At 0 mV, the ion conduction decreases upon DOTAP content increment from 40% to 100% ((d) black square), which overlaps exactly with the synchronized mobility increase of both S4 bottom and pore. The coupling of sensor and the pore of the KvAP leads to the functional difference.
