Supplementary Figure 1: Ci-VSD R217E structure details at 2.5-Å resolution.
From: Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain
(a) The Ci-VSD-R217E+33F12_4 crystal belongs to space group P6522 and contains 12 complexes (6 homodimers) in the unit cell. The dimer is formed with a crystallographically related symmetry partner on a screw axis. (b) 2Fo-Fc electron density maps (σ=1.0) of Ci-VSD-R217E complex. The S4 segment is colored in red. (c) Close-up view of R217E structure. The side chains are well resolved at 2.5 Å resolution throughout Ci-VSD including the gating arginine residues (magenta) R223, R226, R229 and R232. (d) Three LDAO detergent molecules and one succinic acid molecule were resolved around S3-S4 loop region of R217E structure (yellow sticks).
