Supplementary Figure 2: EPR spectra for all scanned residues.
From: Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain

Continuous wave EPR spectra of spin labeled single cysteine Ci-VSD-1-260 mutants on both WT (red) and R217E (black) background scanning the region 201-250. Each pair of spectra was normalized by double integration. The largest difference in spectra shape occurred at the end of the S4-phosphatse linker which is consistent with the heterogeneity of the linker from the crystal structures.