Supplementary Figure 6: Example gels for the Lys48-linked diubiquitin cleavage assay of Rpn11. | Nature Structural & Molecular Biology

Supplementary Figure 6: Example gels for the Lys48-linked diubiquitin cleavage assay of Rpn11.

From: Structure of the Rpn11–Rpn8 dimer reveals mechanisms of substrate deubiquitination during proteasomal degradation

Supplementary Figure 6

Shown are the gels for one Michealis-Menten experiment analyzing the 30-minute time courses of Lys48-linked di-ubiquitin cleavage by wild-type Rpn11-Rpn8 (5 μM) at substrate concentrations between 15 and 500 μM. Bands indicated by asterisks are due to contaminating proteins that co-purify at low abundance with Rpn11 and Rpn8 heterodimers. Covalently linked ClpX hexamer was used for normalization of staining and enzyme concentrations across different gels, which were all processed in parallel.

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