Supplementary Figure 4: Water-accessibility profile of mHv1cc.
From: X-ray crystal structure of voltage-gated proton channel
Comparison of water accessibility profile of mHv1cc with previous findings on mHv1, CiHv1 and hHv1. The symbols “+” and “–” show the accessible and the inaccessible residues, respectively. The information of accessible or inaccessible sites were cited from several studies of the cysteine–scanning with accessibility to MTS or the histidine–scanning of zinc sensitivity by electrophysiology. State–dependent MTS or Zn2+ accessibility are shown with three colored characters (blue; site extracellularly accessible to MTS or Zn2+ in activated–state from CiHv1 or hHv1. red; site intracellularly accessible to MTS in resting–state from CiHv1. green; site intracellularly accessible to Zn2+in activated–state from hHv1). #cysteine scanning of accessibility to MTS reagent done by electrophysiology on CiHv1 (Ref. 19) ¶ histidine scanning of Zn2+ sensitivity done by electrophysiology on hHV1 (Ref. 20) §cysteine scanning of accessibility to MTS reagent done by electrophysiology on CiHv1 (Ref. 18) ‡ accessibility to AMS reagent44,45
