Supplementary Figure 2: Crystal structures and omit maps of αVβ3–FN10 complexes.
From: Structural basis for pure antagonism of integrin αVβ3 by a high-affinity form of fibronectin
Ribbon diagrams of αVβ3 ectodomains bound to wtFN10 (a) or hFN10 (b). Both integrins are in the same orientation. αV chain is in blue and β3 chain is in light green (a) or pink (b). The four-αV domains (Propeller, Thigh, Calf-1 and Calf-2) and eight domains of β3 (PSI, βA, Hybrid [H], IE1-4 and βTD) are marked in (a). Orange spheres represent the five Mn2+ ions at the base of the Propeller and at the genu of αV. Mn2+ ions at LIMBS (gray), MIDAS (cyan) and ADMIDAS (magenta) are shown as spheres. Glycan carbons are indicated in the respective chain color. (c, d) σA weighted Fo-Fc omit electron density (ED) maps of the ligand-binding site and surrounding area for αVβ3-wtFN10 (c) and αVβ3-hFN10 (d) structures contoured at 4.0 σ, with FN10 protein omitted from the map calculation in each case.
