Supplementary Figure 3: CSR-1 affects Pol II transcription at promoters and gene ends and interacts with Pol II.

(a, b) Cumulative distribution plots of normalized GRO-seq promoter and gene end reads (log₂ of the reads' ratios: drh-3(ne4253)/WT) showing the overall effect of drh-3 mutation on Pol II transcription of CSR-1 targets (orange line), and all genes (black line). (c) ChIP-qPCR results in L4- staged worms showing the reduction of Pol II binding at the promoters of CSR-1 target genes in drh-3(ne4253) versus WT. Error bars indicate the range (n = 2 biological replicates). (d) Coimmunopercipitation results with nuclear extracts from WT worms and csr-1 hypomorphic mutant strain show specific interaction between CSR-1 and the Pol II complex. The top panels show western blots with an antibody that recognizes the nonphosphorylated isoform of Pol IIand the bottom panels show western blots with an antibody recognizing the long and the short isoforms of CSR-1. Left panels show immunoprecipitation with CSR-1 antibody and right panels show immunoprecipitation with Pol II antibody. Long and short CSR-1 isoforms are indicated by the arrows.