Supplementary Figure 1: Histone abundance is not compromised in csr-1 hypomorph and drh-3(ne4253) mutant worms.

(a) Venn diagram showing an overlap between genes down-regulated at least 1.5 fold in csr-1(tm892) mutant (by tiling array¹⁵) and CSR-1 target genes¹⁵. A Fisher's exact test was used for calculating a P value for the significance of the overlap. (b) Western blotting showing that the levels of H2B (left panel) and H2A (center panel) in csr-1 hypomorph and the level of H2B in drh-3(ne4253) mutant (right panel) are not reduced compared to the control. Ponceau red staining is shown as a loading control (membrane). (c) Western blot showing the two isoforms of CSR-1 present in the cytoplasmic and nuclear fractions (top panel). In control western blots histone H3 is detected in the nuclear fraction (bottom panel) and actin is detected in the cytoplasmic fraction (middle panel). (d) Western blot showing reduced levels of CSR-1 in nuclear extracts from csr-1 hypomorph compared to the control. Ponceau red staining is shown as a loading control (membrane). (e) ChIP-qPCR results with histone H3 antibody detect no significant changes in the incorporation of histone proteins into chromatin at selected germline-specific CSR-1 targets and nontarget loci in csr-1 hypomorph and drh-3(ne4253) mutants in comparison to WT. Data are presented as the mean of the percentage of input in three independent experiments. Error bars, s.d. (n = 3 biological replicates).