Supplementary Figure 3: Spin labeling of TM6 residues.
From: Conformational dynamics of ligand-dependent alternating access in LeuT
(a) The intracellular network of hydrogen bonding and electrostatic interactions stabilized by Y268A of IL3. (b) The network of spin label pairs monitoring the movement of TM6b and the loop connecting to TM7a is shown by black spheres connected by solid lines. b) Na+- and Leu-dependent changes in the distance distributions demonstrating the movement of TM6b in the WT (solid lines) and the R5A (dashed lines) backgrounds. The broad distributions hinder analysis of the magnitude of distance changes but are consistent with the ligand-dependent equilibrium of this TM between multiple conformations.
