Supplementary Figure 3: WT and GE mutant but not CS mutant of PTEN can dephosphorylate IRS1 in cells. | Nature Structural & Molecular Biology

Supplementary Figure 3: WT and GE mutant but not CS mutant of PTEN can dephosphorylate IRS1 in cells.

From: PTEN is a protein tyrosine phosphatase for IRS1

Supplementary Figure 3

(a)Validation of PTEN shRNA MEFs reconstituted with equal amount of GFP-S-tagged PTEN (WT/CS/GE). (b-e) The experiments were performed the same as that in Figure 4 except that both p-IRS1 (Y608) and p-IRS1 (Y989) were monitored here. (f) PI3K inhibitors block IGF1-induced phosphorylation of AKT but not that of IGF1R or IRS1. WT MEFs were pretreated for 1 hr with either 2 μM BYL-719 or 1 μM GDC0941, followed by 50 ng/ml IGF1 for 10 min.

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