Supplementary Figure 5: NEDD4 is required for AKT activity in MCF7 but not MDA-MB-468 cells. | Nature Structural & Molecular Biology

Supplementary Figure 5: NEDD4 is required for AKT activity in MCF7 but not MDA-MB-468 cells.

From: PTEN is a protein tyrosine phosphatase for IRS1

Supplementary Figure 5

(a) The effect of IGF1R inhibitor, EGFR inhibitor on AKT activity in MCF7 and MDA-MB-468 cells. MCF7 and MDA-MB-468 cells were treated with either 5 μM IGF1Ri or 5 μM EGFRi for 3 hrs. γ-tubulin is used as loading control. (b) The effect of NEDD4 RNAi on AKT activity in MCF7 and MDA-MB-468 cells. MCF7 and MDA-MB-468 cells harboring Dox-inducible NEDD4 shRNA construct were treated with or without 1 μg/ml Dox for 3 days

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