Supplementary Figure 1: NEDD4 specifically regulates IGF but not EGF or serum signaling. | Nature Structural & Molecular Biology

Supplementary Figure 1: NEDD4 specifically regulates IGF but not EGF or serum signaling.

From: PTEN is a protein tyrosine phosphatase for IRS1

Supplementary Figure 1

(a) Western blotting analysis of (AKT phosphorylation) upon different stimuli. NEDD4+/+ and NEDD4-/- MEFs were serum starved for 3 hrs, followed by 5 min stimulation with 10% serum, IGF1 (50 ng/ml), or 50 ng/ml, 100 ng/ml IGF2. β-actin is used as a loading/sample preparation control. (b) Western blotting analysis similar as in (a), with different amounts of EGF; EGFR phosphorylation was also assessed. γ-tubulin is used as loading control. (c) Western blots analyses of time course of the effect of NEDD4 elimination on insulin signaling. MEFs harboring Dox-inducible NEDD4 shRNA construct were treated with or without 1 μg/ml Dox for 3 days, serum-starved for 3 hrs, and then stimulated for the indicated amount of time with insulin (100 ng/ml).

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