Supplementary Figure 2: Structural and biochemical analysis of Pan2–Pan3.
From: The structure of the Pan2–Pan3 core complex reveals cross-talk between deadenylase and pseudokinase
(a) Snapshots of the simulated annealing Fo-Fc omit map countered at 2.5σ (mesh, in grey) for different segments of the Pan2 linker. The anomalous signal peak for the Selenomethionine residues is shown in red, contoured at 4.0σ. Abbreviation: UCH (ubiquitin C-terminal hydrolase). (b) Size exclusion chromatography profile of Pan2fl–Pan3ΔN with standard molecular weight markers. The calculated mass of the complex is shown on the right. Abbreviations: Pan3ΔN (residues 226–679), H10 (His-tag), fl (full length), MW (molecular weight), A280nm (Absorption at 280 nm).
