Supplementary Figure 5: Gene inactivations have different effects on foci accumulation in the nucleus.
From: Identification of genes in toxicity pathways of trinucleotide-repeat RNA in C. elegans
Computational analysis of SM-FISH images of 0CUG animals (yellow dots), control (green dots), and 123CUG animals fed different gene inactivations (blue dots) and control vector (red dots). Each ‘dot’ shown in the graph represents one analyzed SM-FISH image, corresponding to a single imaged cell. The red dotted square indicates the region of clustering of the samples corresponding to 123CUG animals on control vector (red dots). Labeled on the graph on the left, above the red box, are the gene inactivations (blue dots) that cause an increase in bright pixel intensity, corresponding to an increase in foci size or number, relative to the 123CUG on control. The ‘grouping’ of 123CUG npp-4 inactivations in the upper right corner of the graph indicates both an increase in nuclear foci and in nuclear ‘single’ transcript localization relative to the 0CUG npp-4 controls that localize further to the left in the graph. The inset section displayed shows gene inactivations (blue dots) that cause a decrease in bright pixel intensity, relative to the 123CUG on control vector, corresponding to a decrease in foci size or number. Axes correspond to fraction.
