Supplementary Figure 4: FRET analyses of 30S head swiveling. | Nature Structural & Molecular Biology

Supplementary Figure 4: FRET analyses of 30S head swiveling.

From: EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon–anticodon duplex

Supplementary Figure 4

30S head swiveling indicated by changes in FRET efficiencies between donor Alexa 555 and acceptor Alexa 647 probes attached to S11 and S13 for the S11-Donor/S13-Acceptor pair. PRE 70S complexes were rapidly mixed with EF-G wt or the EF-G mutant S588P or Δloop II and GTP at time 0 and Alexa 647 emission was recorded. Each trace represents the average of 10 reactions.

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