Supplementary Figure 2: In vivo experiments of EF-G and the mutants. | Nature Structural & Molecular Biology

Supplementary Figure 2: In vivo experiments of EF-G and the mutants.

From: EF-G catalyzes tRNA translocation by disrupting interactions between decoding center and codon–anticodon duplex

Supplementary Figure 2

(a) Colonies of E. coli cells over-expressing EF-G or its mutants on agar plates. (b) Polysome pattern of the cells in a. Without IPTG treatment (IPTG-), all mutants exhibited similar polysomes pattern as in WT EF-G cell (black curves). When the cells have been induced with ITPG, the polysomes patterns showing difference are indicated in red (strong) and pink (mild).

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