Supplementary Figure 2: Identification of a Sgo1-binding site on SA2–Scc1.
From: Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion
(a,b) Quantification of normalized intensities of the indicated 35S-labeled SA2–Scc1 proteins bound to beads coupled to phospho-T346 Sgo1 peptides. WT, wild type. Error bars, s.d. (n = 3, independent experiments). (c) Anti-Myc, anti-GFP, and anti-Sgo1 blots of lgG and anti-GFP IP from GFP-Sgo1-expressing HeLa cells co-transfected with Scc1-Myc and the indicated Myc-SA2 plasmids. WT, wild type. (d) Anti-Myc, anti-SA2, and anti-tubulin blots of lysates of HeLa cells transfected with the indicated siRNAs and plasmids. WT, wild type. (e) Quantification of mitotic indices (defined as MPM2-positive, 4N cells) of cells in d. Error bars, s.d. (n = 7, independent experiments).
