Supplementary Figure 3: The conserved FVHRYRD motif of SA2 is not required for cohesin loading in human cells.
From: Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion
(a) Cartoon diagram of the structure of human SA2–Scc1, with SA2 colored blue and Scc1 colored pink. The conserved SA2–Scc1 regions implicated in binding the cohesin loader Scc2–Scc4 are colored yellow. The MES molecule bound near the SA2–Scc1 interface is shown in sticks. The chemical structure of MES is shown in the lower right corner. (b) A zoomed-in view of the MES-binding site, with the kicked OMIT map of electron density around MES shown at a contour level of 3σ. (c) DAPI (blue in merge), anti-GFP (red in merge), and anti-tubulin (green in merge) staining of telophase HeLa cells transfected with the indicated GFP-SA2 plasmids and siRNAs. WT, wild type. Scale bar, 10 μm. (d) Quantification of the anti-GFP staining intensities of cells in c. Each dot in the graph represents a single cell (Mock, n = 12; Vector, n = 22; WT, n = 48; Y297A, n = 18; R298E, n = 46; D793K, n = 40). The horizontal bars indicate the means.
