Supplementary Figure 6: Dynamic analysis of chromatin modifiers and corresponding modifications. | Nature Structural & Molecular Biology

Supplementary Figure 6: Dynamic analysis of chromatin modifiers and corresponding modifications.

From: High-temporal-resolution view of transcription and chromatin states across distinct metabolic states in budding yeast

Supplementary Figure 6

(a) shows the numbers of binding sites and annotated genes by MACS for Gcn5, Esa1 and Set1. Areas of overlap among the three enzymes and numbers of annotated genes in each subset are labeled inside the Venn diagram. (b) 4 distinct patterns of the 1035 genes to which all three enzymes bind show the temporal combinatorial recruitment of the chromatin modifiers. Overrepresented GO terms for each cluster are listed on the right. (c) Gcn5-3FLAG 6HA-Set1 cells were collected at 8 time points from late RC to RB phase in YMC for ChIP-qPCR. (d) ChIP-qPCR of H3K9ac, H3K14ac, H4K5ac and H3K4me3 at examples of OX phase genes. RPS11b and RPL33b are two RP genes and MET5 and LYS1 are aa genes. H3K14ac and H4K5ac were detected earlier than H3K9ac on aa genes but not on RP genes. (e) ChIP-qPCR of Gcn5, Set1 and Esa1 at RPS11b and RPL33b. Esa1 and Set1 binding increase before Gcn5 binding.

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