Supplementary Figure 3: Examination of nucleotides with altered reactivity in three SSU intermediates.
Nucleotides of mature 16S rRNA with altered reactivity in the three pre-SSU complexes purified with different tags are shown. The relative changes in the nucleotide reactivity intensity of the intermediates to mature subunits are plotted on a log2 scale (see Supplementary Data Set1). The dotted lines indicate cutoff values for relative change in nucleotide reactivity to be considered. The regions involved in pseudoknot formation, intersubunit bridges and tRNA interactions are marked.
