Supplementary Figure 5: RES-RNA interaction.
From: Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex
(a) 1H, 15N chemical shift perturbation (CSP) imposed on cSnu17p in cSnu17p–cPml1p dimer and in cSnu17p–cBud13p dimer upon titration with CUUCAUCUUUUUG RNA. CSP mapped on the structure of cRES (left of each graph). Significant CSPs were grouped and color-coded into three categories according to: medium (light pink) if 2σ>CSP>1σ, strong (pink) if 3σ>CSP>2σ, very strong (red) if CSP >3σ, were σ is the standard deviation of the mean for the CUUCAUCUUUUUG to cRES titration. Only 224 to 238 residues of cBud13p are shown for clarity. (b) CSP binding curves derived from a representative set of residues experiencing high CSP and residue-averaged dissociation constants (Kd) of CUUCAUUCUUUUUG and all four members of the cRES assembly pathway. (c) Uncropped western blots from main text Fig. 5a. From left to right, Ponceau-stained membrane, peroxidase-anti-peroxidase detection of RES-TAP and autoradiography.
