Supplementary Figure 6: 1H-15N normalized chemical-shift perturbation (CSP) imposed on cSnu17p in cRES upon titration with various RNAs.
From: Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex
(a) NCSP mapped on the structure of cRES (left of each graph). Significant NCSPs were grouped and color-coded into three categories according to: medium (light pink) if 2σ>NCSP>1σ, strong (pink) if 3σ>NCSP>2σ, very strong (red) if NCS >3σ, were σ is the standard deviation of the mean. Only 224 to 238 residues of cBud13p are shown for clarity. The given RNA sequence is indicated above each graph. (b) CSP binding curves derived from a representative set of residues experiencing high CSP upon ACGAAUUAGA titration and average binding affinity (below). * value derived from CSP of two residues.
