Supplementary Figure 2: Validation of the lysine-deficient SUMO-2 mutant and confirmation of new SUMO-2 targets.
From: Uncovering global SUMOylation signaling networks in a site-specific manner
(a) Immunoblot analysis (IB) of total lysates and His10-pulldown samples (PD) from HeLa cells expressing His10-SUMO-2 wild-type (WT) or lysine-deficient (K0) mutant, which were mock treated, or treated with heat shock, PR-619 or MG-132. Ponceau-S staining on total lysates is shown as a loading control. The experiment shown was replicated in biological duplicate.
(b) Immunoblot analysis of total lysates and His10-pulldown samples from HeLa cells or HeLa cells expressing His10-SUMO-2. The experiment shown was performed in biological duplicate.
(c) Similar to (b), but additionally using either mock treated or PR-619 treated cells. PR-619 LFQ ratios are indicated below their respective antibodies. The experiment shown was performed in biological duplicate.
