Supplementary Figure 3: A comparison of SUMOylated proteins and sites identified in this work to proteins and sites previously identified in other SUMOylation studies.

(a) Schematic overview of SUMOylated proteins identified in this study, as compared to previously published studies. From Golebiowski et al.¹, control SUMOylated proteins and heat shock SUMOylated proteins were classed separately. From Bruderer et al.², all SUMOylation proteins and molecular-weight corrected SUMOylated proteins were classed separately. From Becker et al.³, SUMO-2 modified proteins and all SUMO-1 and SUMO-2 modified proteins were classed separately. From Tammsalu et al.⁴, proteins SUMOylated after heat shock were compared with proteins SUMOylated after heat shock in this study, and separately with proteins identified under all other conditions. (b) Schematic overview of SUMOylation sites identified in this study, as compared to Matic et al.⁵, Schimmel et al.⁶, and all known SUMOylation sites from PhosphoSitePlus (PSP). Sites identified after heat shock by Tammsalu et al. were compared to sites identified after heat shock in this study, as well as sites detected under standard growth conditions and sites detected after MG-132 or PR-619 treatment in this study.