Supplementary Figure 7: Expression levels of Atxn7, lnc-SCA7, miR-124 and targets in SCA7 mice.

Fold difference in normalized expression (relative to Gadph) measured by qRT-PCR of miR-124 (dark grey), lnc-SCA7 (blue) and ATXN7 (red) across tissues derived from SCA7 mouse models relative to matched controls (white): (A) Retina and (B) cerebellum of 28 weeks SCA7^100Q/100Q mice, and (C) retina, (D) cerebellum, (E) cortex, (F) striatum, (G) olfactory bulb and (H) spinal cord of SCA7^266Q/5Q animals. (I and J) The fold-difference (y-axis) in expression in the (I) cerebellum or (J) retina of 5 week SCA7^266Q/5Q mice (grey) relative to matched littermate SCA7^5Q/5Q control mice (white) were elevated for 8 and 12 (of 13) known miR-124 targets (light grey), respectively. Relative pri-miR-124a1 (dark grey) levels were reduced in these tissues in SCA7 mice. Fold difference in normalized expression (relative to Gadph) measured by qRT-PCR of miR-124 (dark grey), lnc-SCA7 (blue) and ATXN7 (red) across tissues derived from two SCA7 mouse models relative to controls. (K, L) livers, (M, N) lungs and (O) muscle of 28 week SCA7^100Q/100Q or 5 week SCA7^266Q/5Q mice relative to matched control mice (SCA7^5Q/5Q, white). Error bars represent s.d.m. n = 3 cell cultures per condition; * p < 0.05; ** p < 0.01; ***,p < 0.001; Not significant, NS; Two-tailed Student’s t-test.