Supplementary Figure 3: The ability of lnc-SCA7 to regulate Atxn7 expression is dependent on miR-124 but not on miR-16. | Nature Structural & Molecular Biology

Supplementary Figure 3: The ability of lnc-SCA7 to regulate Atxn7 expression is dependent on miR-124 but not on miR-16.

From: Cross-talking noncoding RNAs contribute to cell-specific neurodegeneration in SCA7

Supplementary Figure 3

Sequence alignment of (A) miR-124 and (B) miR-16 and their respective miRNA response elements (MREs) within the 3’ UTRs of mouse lnc-SCA7 (top panels) or Atxn7 (bottom panels). The initial base of the predicted binding sites in these transcripts is noted to the left of the alignment. Identical nucleotides between miR-124/miR-16 with lnc-SCA7 and Atxn7 are denoted by vertical lines. (C) Effect of over-expression of miR-16 mimics (top right box, light blue) in N2A cells on the expression levels of lnc-SCA7 (NS, blue) or Atxn7 (NS, red) relative to a non-specific sequence used as control (white). (D) miR-16 (light blue) is relatively more lowly expressed in N2A cells compared to miR-124 (dark grey). Error bars represent s.d.m.; n = 3 cell cultures per condition; *** p < 0.001; Two-tailed Student’s t-test.

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