Supplementary Figure 4: U2AF65 binding profile on representative genes and insertion mutational analysis of U2AF65-binding site–induced changes in alternative splicing.
From: Mechanisms for U2AF to define 3′ splice sites and regulate alternative splicing in the human genome
(a) U2AF65 binding on the alternative exon in GANAB. (b) U2AF65 binding on the alternative exon in ANKRD10. PCR-validated splicing changes are shown in the right. (c) The minigene constructs illustrating the insertion of the U2AF65 binding site from an intronic region in DROSHA (see Fig. 4d) into an U2AF65 insensitive gene C1orf43 either in an upstream or a downstream intronic location. Bottom panel: RT-PCR analysis of wt and mutant minigenes in response to U2AF65 RNAi.
