Supplementary Figure 5: Similar effects of U2AF65 RNAi and U2AF35 RNAi on alternative splicing.
From: Mechanisms for U2AF to define 3′ splice sites and regulate alternative splicing in the human genome
(a) Examples of induced exon skipping events in response to U2AF65 or U2AF35 RNAi. (b) Examples of induced exon inclusion events in response to U2AF65 or U2AF35 RNAi. (c) Concordant splicing response to U2AF65 and U2AF35 RNAi determined by RT-PCR. ∆PSI: Difference of Percentage of Splicing In (PSI) between HeLa cells treated with U2AF35 or U2AF65 siRNA and negative control (NC) siRNA. (d) Heatmap showing similar U2AF65 RNAi-induced splicing events with or without exogenously expressed U2AF35. (e) RT-PCR validation of a set of U2AF65 RNAi-induced splicing events in the presence or absence of overexpressed U2AF35.
