Supplementary Figure 1: Mapping of U2AF65 CLIP tags to the human genome.
From: Mechanisms for U2AF to define 3′ splice sites and regulate alternative splicing in the human genome
(a) Western analysis of the IP efficiency of anti-U2AF65 antibody. (b) Comparison between U2AF65 CLIP-seq data generated in the current study with the published U2AF65 iCLIP-seq data17. (c,d,e) Profiles of base deletion (c), insertion (d) and substitution (e) detected by CIMS analysis of U2AF65-RNA interactions37. (f) Preferential deletion mutation on uridine residues in CIMS. (g) Footprint of U2AF65 binding on RNA based on CIMS.
