Supplementary Figure 6: Local resolution maps and FSC curves of the three nonrotated 80S–RAC density maps.
From: Structural basis for interaction of a cotranslational chaperone with the eukaryotic ribosome
(a-e, g-k, and m-q) Local resolution maps of the 4.9 Å (a-e), 5.2 Å (g-k) and 7.2 Å (m-q) density maps of the nonrotated 80S-RAC complex are colored according to the color scale on the right. The maps are displayed in classical front view (a-b, g-h and m-n) and a viewed centered on RAC (c-e, i-k, and o-q). Respective central slices of the local resolution maps are also shown (b, d, h, j, n, and p). Due to the substoichiometric binding and the flexibility of factors, local resolution maps are displayed at low contour levels (e, k and q), in order to show factor densities. As shown, with the increase of sample homogeneity (from e, k to q), local resolution at the factor region improves as well, although the overall resolution of the density maps becomes worse. (f, l and r) Gold-standard fourier shell correlation (FSC) curves of the three density maps.
