Supplementary Figure 6: In vitro transcription assays on chromatin templates.
From: BRD4 assists elongation of both coding and enhancer RNAs by interacting with acetylated histones
a, Diagram showing protocol for the in vitro transcription assays on chromatin templates. In vitro transcription elongation assays were performed on a hypo-acetylated or hyper-acetylated chromatin pG5MLP template, using recombinant FACT or BRD4; together with general transcription factors (GTFs) and RNA polymerase II. b, Relative quantification of histone H3 modifications by quantitative mass spectrometry in hypo-acetylated and hyper-acetylated nucleosomes. Results of two independent measurements are shown.
