Supplementary Figure 8: BRD4 is associated with Pol II elongation complexes in vivo.
From: BRD4 assists elongation of both coding and enhancer RNAs by interacting with acetylated histones
a, Validation of native RIP (RNA-Immunoprecipitation). Native nuclear complexes containing YFP-BRD4short and RNA were released by DNaseI digestion, and immunoprecipitated with anti-YFP antibody (for BRD4short) or control IgG, followed by extraction of co-precipitated RNA. Quantitative PCR using Klf4 specific primers was performed with or without reverse transcription (RT). b, Quantitative RT-PCR analysis of the RunOn-RIP signals along the Myc gene locus. Background signals were assessed using normal rabbit IgG. c, Genome browser view of RunOn-RIP-seq reads around the Myc gene locus, representing the actively elongating front of transcripts that was associated with YFP-BRD4short.
