Supplementary Figure 8: Controls for TR-SAXS measurements.
(a,b) Time-resolved SAXS (TR-SAXS) experiments of cIAP1-B3R and cIAP1-B3RL617E mixed with buffer instead of AVPW. (c, d) TR-SAXS experiment of cIAP1-B3R-∆C7 mixed (c) with buffer and (d) with AVPW. (e) TR-SAXS analysis of cIAP1-B3RL617E with a higher concentration of AVPW (1 mM). The relative populations of the monomeric, open, and dimeric conformations of cIAP1 were extracted from the TR-SAXS data by deconvolution using the static scattering curves of each state as reference (See Methods). Each point represents and average from three experiments, plus or minus standard deviation. Data are fit to first order integrated rate equations. The closed monomer fraction is shown as blue squares, open fraction as hot pink circles, dimer fraction as purple diamonds.
