Supplementary Figure 3: Methionine methyl groups assigned by mutagenesis.

1H-13C HMQC spectra of each methionine to leucine point mutant (blue) overlaid with the cIAP1-B3R spectrum (red). The M466L mutant is also shown with the contours drawn four times lower. The M266L, M391L, M392L, and M402L spectra display secondary chemical shift changes that may be due to structural perturbations as a result of the mutation.