Supplementary Figure 1: Amino acid sequences of the LepB construct, the test segments and the SecM arrest-peptide variants used in this study.
From: Charge-driven dynamics of nascent-chain movement through the SecYEG translocon
(a) The amino acid sequence of the full-length LepB construct is shown with the wild type 8-residue M. succiniciproducens SecM arrest peptide (bold) and 5D test segment (bold and underlined). The two natural TM domains of LepB (TM1, TM2) are underlined. The last residue indicated by the left-facing arrow (denoted ‘R’) was fused to the first residue indicated by the right-facing arrows of downstream LepB residues to give constructs with linker lengths, L, indicated by the numbers above the arrows.
(b) Sequences of the test segments analyzed in this study. All test segments used have GG...GG flanks, except for the SG constructs which have (SG)5...(SG)5. flanks and the [6L,13A] segment which has GGPG...GPGG flanks.
(c) Sequences of SecM arrest peptide variants used. Mutations introduced into the wild type SecM arrest peptide to generate the non-functional variant (mut) and the stronger variant (Sup1) are underlined.
