Supplementary Figure 4: Effect of parental gene knockdown and circRNA overexpression.
From: Exon-intron circular RNAs regulate transcription in the nucleus
(a-c) Knockdown of EIF3J or PAIP2 mRNA with shRNA plasmids or siRNA had no effect on the level of circRNA. The diagrams below the bar figures show the positions of the shRNA- or siRNA-targeted sites in EIF3J or PAIP2. (d and e) Overexpression of circEIF3J or circPAIP2 with plasmids had no effect on the level of EIF3J or PAIP2 mRNA in HeLa (d) and HEK293 cells (e). All data were from triplicate experiments. Error bars represent the s.e.m. *, P value < 0.05, **, P value < 0.01. P values were determined with the two-tailed Student’s t-test.
