Supplementary Figure 8: ChIP assay results.
From: Exon-intron circular RNAs regulate transcription in the nucleus
(a) Flow chart of the experimental setup for pol II, U1A, and U1C ChIP after siRNA knockdown. (b) Semi-quantitative PCR corresponding to the real-time PCR results shown in Fig. 6e. (c) Association between pol II (pol II ChIP) and the gene body decreased upon siRNA-mediated knockdown of circRNA. Real-time PCR results are shown, and the sites examined within the gene body are the same as those shown in Supplementary Figure. 6b. All data are from triplicate experiments. Error bars represent the s.e.m. *, P value < 0.05, and **, P value < 0.01. P values were determined with a two-tailed Student’s t-test. (d) siRNA-mediated knockdown of circEIF3J had no effect on the association between pol II and the PAIP2 gene body or vice versa. (e) Binding of pol II to parental gene promoters was not altered by knockdown of parental mRNA. The sites examined within the gene promoter are the same as those shown in Fig. 5d.
