Supplementary Figure 1: Local and global resolution statistics for the 40S–eIF1–eIF1A–eIF3–eIF3j complex.
From: Structure of a Yeast 40S–eIF1–eIF1A–eIF3–eIF3j initiation complex
Fourier shell correlation for 40S–eIF1–eIF1A–eIF3–eIF3j calculated between two independently refined half sets; resolution cut-off indicated by the red line (left panel). The “gold-standard” Fourier shell correlation was determined, and resolution estimated, according to the method of S. Scheres37. The surface of the 40S–eIF1–eIF1A–eIF3–eIF3j reconstruction low-pass filtered at global resolution (6.47 Å) is shown coloured according to the local resolution (centre and right panels – 5 Å shown in blue through to 15 Å shown in red). The mean local resolution was 12.9 Å for eIF3ac, 9.5 Å for eIF3a CTD, 10.3 Å for eIF3b WD40, 14.7 Å for eIF3gi, 14.0 Å for eIF3b RRM and 10.8 Å for eIF1–1a–3j. Local resolution was determined using the implementation of Kucukelbir and colleagues: ResMap38.
