Supplementary Figure 3: YopO mutants.
(a) Disrupting the YopO:actin interface in YopO nAct. YopO is colored as in Fig. 1a. Panels show close up of the interfaces between YopO and actin at the mutation sites, which were used to disrupt the interactions with actin in YopO nAct. Mutations V374D and T376D introduce large charged groups into a hydrophobic YopO:actin interface while mutations R723A and E727A disrupt electrostatic interactions between YopO and actin.
(b) Size exclusion chromatograms of YopO WT and mutants. YopO WT and mutants have a similar rate of migration on size exclusion chromatography, suggesting that the mutations do not disrupt the normal folding of YopO. WT, KD, nAct and nRac denote wild type, kinase-dead, non-actin binding and non-Rac binding respectively.
