Supplementary Figure 1: Characterization of zCYLDΔBbox.
From: Structures of CYLD USP with Met1- or Lys63-linked diubiquitin reveal mechanisms for dual specificity
(a) Linkage specificity of zCYLDΔBbox. Ub2 species (10 μM) were incubated in the presence of 10 μM zCYLDΔBbox at 37 °C for 5 and 90 min and analyzed by tricine SDS-PAGE with Coomassie brilliant blue staining.
(b) Superposition of zCYLDΔBbox in the Met1 catalytic state and hCYLD (containing B-box) in the apo state (PDB:2VHF). The drawing scheme is the same as that in Fig. 1. The USP domain and the B-box domain of hCYLD are colored orange and yellow, respectively. In the Met1 catalytic state, both Ubdist and Ubprox are more than 12 Å from the likely position of the B-box domain, as estimated from the apo structure of the hCYLD USP domain. This is consistent with the B-box domain being dispensable for Met1- and Lys63-linkage-specific DUB activities of CYLD.
