Supplementary Figure 2: Kinetic analyses of zCYLDΔBbox, Met1- and Lys63-Ub2 mutants.
From: Structures of CYLD USP with Met1- or Lys63-linked diubiquitin reveal mechanisms for dual specificity
Plots of initial velocity as a function of Met1- or Lys63-Ub2 concentration. Error bars represent standard deviations in three independent experiments. The data were fitted to the Michaelis-Menten kinetic model.
(a) Cleavage of Met1-Ub2 by mutant zCYLDΔBbox
(b) Cleavage of mutant Met1-Ub2 by zCYLDΔBbox
(c) Cleavage of Lys63-Ub2 by mutant zCYLDΔBbox
(d) Cleavage of mutant Lys63-Ub2 by zCYLDΔBbox
(e) DUB activity (kcat/KM) of mutant zCYLDΔBbox and Met1- and Lys63-Ub2. The experiments were carried out three times for each mutant except the Q711K mutant of zCYLDΔBbox (twice). Data are presented as mean ± SD. N.D. indicates non-detectable level.
