Supplementary Figure 6: Ago1 L317A protein does not complement ago1Δ but does not show a significant reduction in loading of pericentromeric siRNAs.
From: Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex
(a) Tenfold serial dilutions of otr1R::ura4+ or imr1R::ura4+ pericentromeric silencing reporter cells of the indicated genotypes plated on non-selective medium or medium containing 5-FOA. (b) Western blot analysis of whole cell extracts and FLAG immunoprecipitates prepared from wild-type cells transformed with the indicated 3xFLAG-ago1 overexpression plasmids, and Northern blot analysis of RNA extracted from each immunoprecipitated sample.
